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Cell Applications Inc
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ATCC
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Expression Systems Inc
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Image Search Results
Journal: Journal of visualized experiments : JoVE
Article Title: Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
doi: 10.3791/59010
Figure Lengend Snippet: Quantities of plasmids and reduced serum cell culture medium required to transfect one well of a 6-well plate of HEK-293T/17 cells for pseudotyped particle production. From the concentration of plasmid preparations, calculate the required volume to reach the required amount of plasmid DNA. If more than one well is transfected with the same plasmids, multiply volumes by required number of wells to transfect, and include an extra well in calculations to avoid running out of mix in later steps. The total amount of DNA being transfected is 1 μg/well. Plasmids are available upon request to authors.
Article Snippet: Mix calculated volumes of plasmids and reduced
Techniques: Cell Culture, Concentration Assay, Plasmid Preparation, Transfection, Luciferase
Journal: Journal of visualized experiments : JoVE
Article Title: Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
doi: 10.3791/59010
Figure Lengend Snippet: Table of Materials
Article Snippet: Mix calculated volumes of plasmids and reduced
Techniques: Transfection, Modification, Saline, Cell Counting, Sterility, Luciferase, Lysis
Journal: Journal of visualized experiments : JoVE
Article Title: Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
doi: 10.3791/59010
Figure Lengend Snippet: Quantities of transfection reagent and reduced serum cell culture medium required to transfect one well of a 6-well plate of HEK-293T/17 cells for pseudotyped particle production. Multiply volumes by required number of wells to transfect and include extra wells in calculations to avoid running out of mix in later steps. The transfection reagent: plasmid DNA ratio used is 3:1.
Article Snippet: Mix calculated volumes of plasmids and reduced
Techniques: Transfection, Cell Culture, Plasmid Preparation